Abstract: Dominance hierarchy is often established via repeated agonistic encounters where consistent winners are considered dominant. Human body odour contains cues to psychological dominance and competition, but it is not known whether competition outcome (a marker of a change in dominance hierarchy) affects the hedonic quality of human axillary odour. Therefore, we investigated the effect of winning and losing on odour quality. We collected odour samples from Mixed Martial Arts fighters approximately 1 h before and immediately after a match. Raters then assessed samples for pleasantness, attractiveness, masculinity and intensity. We also obtained data on donors' affective state and cortisol and testosterone levels, since these are known to be associated with competition and body odour quality. Perceived body odour pleasantness, attractiveness and intensity significantly decreased while masculinity increased after a match irrespective of the outcome. Nonetheless, losing a match affected the pleasantness of body odour more profoundly, though bordering formal level of significance. Moreover, a path analysis revealed that match loss led to a decrease in odour attractiveness, which was mediated by participants’ negative affective states. Our study suggests that physical competition and to some extent also its outcome affect the perceived quality of human body odour in specific real-life settings, thus providing cues to dominance-related characteristics.
5. Discussion
In this study, we tested the effect of winning and losing a physical confrontation on human body odour quality and investigated factors that may mediate observed changes. We expected that winning would have a positive effect on body odour quality and losing a negative effect on it. We also predicted that winning would be associated with positive affective states, a rise in T levels and decrease in C levels, while losing would be characterized by the opposite pattern. Moreover, we hypothesized that these factors would mediate a change in body odour quality.
We found that after a match, perceived odour pleasantness, attractiveness and intensity significantly decreased, while masculinity increased in both winners and losers. Nevertheless, the decrease in pleasantness was more pronounced in losers, which suggests that losing has a more negative effect on body odour quality. We found no statistically significant differences in body odour ratings between winners and losers either before or after a match. Path analysis showed that a loss led to a decrease in odour attractiveness and this effect was mediated by participants' negative emotions.
Winning a match may entail a rise in the dominance hierarchy, while losing lowers the rank: in our case, the match score was added to a personal profile, which affects the league rank. Both losing and winning have consequences for both competitors. Nonetheless, the relatively more profound odour changes in losers suggest that losing may be perceived as having more serious consequences because it may be linked not only to lower league ranking but also to more or less serious injuries. In ring-tailed lemurs (Lemur catta), injury affects the chemical composition of secretion and injury status can be discerned by other individuals [86,87]. Similarly, it could be expected that fighters who lost suffered more injuries during a match and that might be reflected in their body odour. In our study, however, that does not seem to be the case since self-assessed pain did not significantly differ between winners and losers and it was not associated with any of the rated odour characteristics.
Our results thus indicate that competitive situations per se can have an effect on body odour, because after a match, while masculinity increased, both pleasantness and attractiveness decreased. The influence of competition on body odour has been demonstrated already by Adolph et al. [37], although they found that the tested odour samples affected exposed individuals on a subliminal level without any perceived differences between competition-linked and control odours. In our study, the perceived odour quality changed, which could be linked to increased sweating owing to the physical effort during the match. Thermoregulation involves mainly eccrine glands [88], which during exertion produce more diluted, watery, and less oily secretion consisting mostly of water and electrolytes derived from blood plasma and sodium chloride [89]. The change in odour quality we observed could thus be owing to the different quality of sweat. This finding should raise caution with respect to using sports odours as control stimuli (e.g. [90,91]), because they may have somewhat different properties than for example odours collected during sleep (e.g. [83]).
(a) Link between dominance and body odour
The results of some animal studies suggest dominant individuals may have specific intrinsic odour qualities [27] that are probably androgen-dependent [43]. In our study, however, ratings of pre-match body odour were similar for winners and losers, suggesting no prior differences in fighters’ body odour quality. Still, we obtained data on the result of only one match and have no information about the fighters' current rank position. We cannot, therefore, draw stronger conclusions about possible links between various qualities of body odour and rank in the dominance hierarchy. In other words, we do not know whether there exists any connection between dominance rank and specific odour qualities, although previous studies did find some olfactory cues to psychological dominance [31,33].
(b) Sex-related differences in rating
The absence of significant sex-linked differences in raters’ assessments in most rated characteristics suggests that these odour cues have no specific role in intra- or intersexual selection. The sole exception was masculinity ratings. Overall, losers' post-match body odour was rated as more masculine than their odour before a match. This effect was driven by male raters who evaluated losers’ body odour as more masculine after a match. Females did not perceive any difference between pre- and post-match quality. Comparison between the calculated POS values and the actual number of male raters, however, indicates a lower stability in masculinity rating by men, which is why this result should be interpreted rather cautiously. Future studies should further investigate these findings to assess their robustness.
(c) The mediating effect of affective states and hormone levels on body odour quality
If we view winning and losing a fight as a way of change in an individual's rank in dominance hierarchy, then a change of position can be reflected in body odour and others can use it to assess the result of competition. This effect could be owing to changes in hormone levels (T and/or C) associated with victory or defeat. This effect has been repeatedly shown in previous studies (e.g. [60,62]). T stimulates the proliferation of sebocytes and affects the function of apocrine sweat glands [92]. Similarly, C has an either direct or indirect link (via adrenaline) with the activity of apocrine glands [93], which can, in turn, affect the quality of body odour. Two studies that investigated the association between T and C levels and body odour quality produced rather contradictory results. Rantala et al. [72] found no significant correlation between T levels and either intensity or attractiveness of men's body odour. Interestingly, however, they found that C concentrations were positively associated with odour attractiveness, though not its intensity. Using a larger sample and a slightly different methodology (a longer sampling period and elimination of samples from men who reported violations of instructions on the use of fragranced cosmetics), Thornhill et al. [94] observed that women in the fertile phase of their menstrual cycle preferred body odours of men with higher T levels than women in the non-fertile phase. This study did not, however, find any significant relation between odour preferences and C levels.
Our study showed no significant effect of competition outcome on T levels. C levels, however, were higher after a match, which suggests that a match is a stressful event for both winners and losers. The positive relationship between C levels and perceived odour attractiveness, shown by mediation analysis, is in line with a previous study by Rantala et al. [72]. Similarly, we found that C levels did not significantly correlate with intensity ratings (a marker of the amount of perspiration), which indicates a qualitative, not quantitative effect. Glucocorticoids possess certain immunosuppressive qualities [95,96] and it has been hypothesized that only immunocompetent individuals can maintain high C levels [97], which is why such preference would be adaptive.
Another set of variables that have previously been linked to both competition outcomes and body odour are affective states. Body odour can contain cues to affective states such as happiness [98], disgust [99], fear [100], anxiety [90], stress [91], but also aggression [38] and contexts such as competition [37]. This is why we investigated possible mediating effects of changes in hormone levels and affective states. A path analysis showed not only a direct effect of competition outcome on perceived odour attractiveness, where a loss led to decrease in odour attractiveness, but also a mediating indirect effect of negative affective state, where the more negative emotions participants experienced, the less attractive was their perceived body odour. Exploratory correlation analysis showed further relationships between some odour characteristics, affective states and hormone levels, which are in line with the evidence summarized above. These findings, however, should be interpreted with caution owing to our relatively small donor sample.
(d) Study limitations
It is a common procedure in body odour studies to ask odour donors to avoid activities that could affect body odour quality, such as eating spicy food, using soaps, deodorants, demanding physical activities, smoking etc. the day before and on the day of sampling (e.g. [83,31]). Owing to logistical reasons, we were able to contact study participants only a few hours before the match. They did not, therefore, receive any such list of restrictions and followed their habitual regime without any standardization as to diet, smoking, shaving, the use of fragranced cosmetics or dietary supplements. This may have introduced some noise to the data and obscured some effects. Nevertheless, we were still able to detect some significant changes and the use of a within-subject experimental design should diminish inter-individual differences. Such an approach has a higher ecological validity, although future studies should investigate the issue under more standardized settings as well.
We explored the possible effect of injuries on body odour quality using a single question regarding participants' self-perceived pain as a proxy for suffered injuries. Admittedly, a record of actual injuries would be a more objective measure, because participants’ answers may be biased by competitiveness and excitement induced by the competition. These data were not, however, available and we did not want to overload participants with additional tasks and screenings.
In the present study, we used hedonic ratings to assess changes in body odour quality induced by winning or losing a match. Apart from explicit subjective ratings, future studies could employ some objective psychophysiological measures such as skin conductance, heart rate, possible activation of different brain areas by fMRI or variance in time activation by EEG.
Finally, future studies should collect data from the same individual both after a victory and a loss in a competition to assess the effect of outcome on body odour directly. In our field study conditions, such balanced design could not be implemented. It therefore remains as a challenge for future research.
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